Review



anti p mlc2  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Cell Signaling Technology Inc anti p mlc2
    Anti P Mlc2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1391 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+p+mlc2/Phospho-Myosin+Light+Chain+2+(Ser19)+Antibody/pm41832874-83-28-30
    Average 96 stars, based on 1391 article reviews
    anti p mlc2 - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    other:

    Article Title: Imidacloprid increases intestinal permeability by disrupting tight junctions.
    Article Snippet: The antibodies used were anti-ZO-1 (ZO-1, Immunoway, YM1410, 1:1000), anti-occludin (occludin, Abcam, ab167161, 1:50,000), anti-claudin-1 (claudin-1, Invitrogen, 374988, 1:4000), anti-caspase 3 (caspase 3, Proteintech, 19677-1-AP, 1:1000), anti-MLC2 (MLC2, Abcam, ab92721, 1:2000), anti-p-MLC2 (p-MLC2, Cell Signaling Technology, 3671, 1:1000), anti-MLCK (MLCK, Abcam, ab76092, 1:5000), anti-NF-κB p65 (NF-κB p65, Cell Signaling Technology, 8242, 1:1000), anti-phosphoNF-κB p65 (phospho-NF-κB p65, Immunoway, YP0191, 1:1000), and anti-PXR (PXR, Immunoway, YT3911, 1:1000).

    Article Title: Platelet-Derived PDGFB Promotes Recruitment of Cancer-Associated Fibroblasts, Deposition of Extracellular Matrix and Tgfβ Signaling in the Tumor Microenvironment
    Article Snippet: The following antibodies and concentrations were used: anti-collagen 1 (2 mg/mL, Abcam 34710), anti-fibronectin (1 μg/mL, Abcam 2413, Cambridge, MA, USA), anti-PDGFRɑ (10 μg/mL, AF1062, R&D, Minneapolis, MN, USA), anti-p-MLC2 (2 μg/mL, Cell signaling 3671, Danvers, MA, USA), anti-p-Smad2 (10 μg/mL, AB3749-I, Millipore, Burlington, MA, USA), anti-cleaved Caspase-3 (1:250, Cell signaling 9661), anti-Phospho-Histone H3 (1:200 μg/mL, Cell Signaling 9701), anti-Ki67 (1 μg/mL, Abcam 15580), anti-rabbit Alexa 555 (2 μg/mL, A31572, Thermo Fisher Scientific, Waltham, MA, USA), anti-rabbit Alexa 488 (2 μg/mL, A21206, Thermo Fisher Scientific), anti-goat Alexa 555 (2 μg/mL, A21432, Thermo Fisher Scientific), anti-rabbit Alexa 647 (2 μg/mL, A21443, Thermo Fisher Scientific).

    Article Title: Glycine alleviates the intestinal epithelial barrier damage induced by imidacloprid via promoting metabolic detoxification.
    Article Snippet: The antibodies used were anti-ZO 1 (ZO 1, Proteintech, 10494–1-AP, 1:5000), anti-occludin (occludin, Servicebio, GB111401–100, 1:5000), anti-claudin 1 (claudin 1, Abcam, ab307692 1:5000), anti-MLC2 (MLC2, Abcam, ab92721, 1:2000), anti-P-MLC2 (P-MLC2, Cell Signaling Technology, 3671, 1:1000), anti-MLCK (MLCK, Abcam, ab76092, 1:5000), anti-NF-κB P65 (NF-κB P65, Cell Signaling Technology, 8242, 1:1000), anti-P-NF-κB P65 (P-NF-κB P65, Immunoway, YP0191, 1:1000), and anti-PXR (PXR, Immunoway, YT3911, 1:1000).

    Article Title: Platelet-Derived PDGFB Promotes Recruitment of Cancer-Associated Fibroblasts, Deposition of Extracellular Matrix and Tgfβ Signaling in the Tumor Microenvironment.
    Article Snippet: The following antibodies and concentrations were used: anti-collagen 1 (2 mg/mL, Abcam 34710), anti-fibronectin (1 μg/mL, Abcam 2413, Cambridge, MA, USA), anti-PDGFRα (10 μg/mL, AF1062, R&D, Minneapolis, MN, USA), anti-p-MLC2 (2 μg/mL, Cell signaling 3671, Danvers, MA, USA), anti-p-Smad2 (10 μg/mL, AB3749-I, Millipore, Burlington, MA, USA), anti-cleaved Caspase-3 (1:250, Cell signaling 9661), anti-Phospho-Histone H3 (1:200 μg/mL, Cell Signaling 9701), anti-Ki67 (1 μg/mL, Abcam 15580), anti-rabbit Alexa 555 (2 μg/mL, A31572, Thermo Fisher Scientific, Waltham, MA, USA), anti-rabbit Alexa 488 (2 μg/mL, A21206, Thermo Fisher Scientific), anti-goat Alexa 555 (2 μg/mL, A21432, Thermo Fisher Scientific), anti-rabbit Alexa 647 (2 μg/mL, A21443, Thermo Fisher Scientific).

    Incubation:

    Article Title: Downregulation of connexin 43-based gap junctions underlies propofol-induced excessive relaxation in hypertensive vascular smooth muscle cells.
    Article Snippet: .. After the cells were blocked with 5% BSA for 30 min, they were incubated overnight at 4 °C with primary antibodies diluted in PBS, including anti-p-MLC2 (#95777S, Cell Signaling Technology, 1:600), anti-MLC2 (#340279, Proteintech, 1:300), and anti-Cx43 (#10906–1-AP, ZEN-BIOSCIENCE, 1:200). .. Then, the cells were further incubated with Alexa Fluor 594-conjugated anti-rabbit secondary antibody (#550043, ZEN-BIOSCIENCE,1:300) or Alexa Fluor 488-conjugated anti-rabbit secondary antibody (#4412S, Cell Signaling Technology, 1:300) in PBS for 1 h at room temperature and in the dark before being exposed to DAPI for 3 min. Three different fields were captured in every experiment by a fluorescence microscope equipped with a 470 nm emission/525 nm emission filter set, a digital CCD camera and a 40 × objective (Thermo Fisher Scientific).

    Article Title: Phosphorylated CPI-17 and MLC2 as Biomarkers of Coronary Artery Spasm–Induced Sudden Cardiac Death
    Article Snippet: .. The tissue sections were blocked in goat serum at 37 °C for 40 min and then incubated with the following primary antibodies overnight at 4 °C: anti-p-CPI-17 (1:100, AF3473, Affinity Biosciences, Cincinnati, OH, USA) and anti-p-MLC2 (1:50, 3671, Cell Signaling Technology, Boston, MA, USA). ..

    Article Title: Downregulation of connexin 43-based gap junctions underlies propofol-induced excessive relaxation in hypertensive vascular smooth muscle cells
    Article Snippet: .. After the cells were blocked with 5% BSA for 30 min, they were incubated overnight at 4 °C with primary antibodies diluted in PBS, including anti-p-MLC2 (#95777S, Cell Signaling Technology, 1:600), anti-MLC2 (#340279, Proteintech, 1:300), and anti-Cx43 (#10906–1-AP, ZEN-BIOSCIENCE, 1:200). .. Then, the cells were further incubated with Alexa Fluor 594-conjugated anti-rabbit secondary antibody (#550043, ZEN-BIOSCIENCE,1:300) or Alexa Fluor 488-conjugated anti-rabbit secondary antibody (#4412S, Cell Signaling Technology, 1:300) in PBS for 1 h at room temperature and in the dark before being exposed to DAPI for 3 min. Three different fields were captured in every experiment by a fluorescence microscope equipped with a 470 nm emission/525 nm emission filter set, a digital CCD camera and a 40 × objective (Thermo Fisher Scientific).



    Similar Products

    96
    Cell Signaling Technology Inc anti p mlc2
    Anti P Mlc2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+p+mlc2/Phospho-Myosin+Light+Chain+2+(Ser19)+Antibody/pm41832874-83-28-30
    Average 96 stars, based on 1 article reviews
    anti p mlc2 - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc rabbit anti p mlc2
    Rabbit Anti P Mlc2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+p+mlc2/pmc13062005-393-8-11
    Average 86 stars, based on 1 article reviews
    rabbit anti p mlc2 - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    95
    Cell Signaling Technology Inc p mlc2
    Inhibition of MLCK signaling mimicked the effects of DHA and EPA on the proportion of zipper-like junctions in PA-treated LECs. (A-D) Effects of ML-7 (50 μM) treatment on the proportion of zipper-like junction proportion (A, B) and the expression of MLCK, <t>p-MLC2</t> (C, D) in PA-treated (100 μM) LECs. White dashed line: VE-cadherin-negative junctions; white arrow: button-like junctions; yellow arrow: zipper-like junctions. Two-tailed unpaired Student’s t -test was performed to compare PA versus PA + DHA/EPA/ML-7-treated groups at the same time points. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
    P Mlc2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+p+mlc2/Phospho-Myosin+Light+Chain+2+(Thr18%2FSer19)+Rabbit+mAb/pmc12869197-93-24-25
    Average 95 stars, based on 1 article reviews
    p mlc2 - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    Image Search Results


    Inhibition of MLCK signaling mimicked the effects of DHA and EPA on the proportion of zipper-like junctions in PA-treated LECs. (A-D) Effects of ML-7 (50 μM) treatment on the proportion of zipper-like junction proportion (A, B) and the expression of MLCK, p-MLC2 (C, D) in PA-treated (100 μM) LECs. White dashed line: VE-cadherin-negative junctions; white arrow: button-like junctions; yellow arrow: zipper-like junctions. Two-tailed unpaired Student’s t -test was performed to compare PA versus PA + DHA/EPA/ML-7-treated groups at the same time points. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Journal: Journal of Advanced Research

    Article Title: Fish oil reduces intestinal fat absorption by promoting lacteal junction zippering via GPR120-VEGFR3-MLCK pathway

    doi: 10.1016/j.jare.2025.05.026

    Figure Lengend Snippet: Inhibition of MLCK signaling mimicked the effects of DHA and EPA on the proportion of zipper-like junctions in PA-treated LECs. (A-D) Effects of ML-7 (50 μM) treatment on the proportion of zipper-like junction proportion (A, B) and the expression of MLCK, p-MLC2 (C, D) in PA-treated (100 μM) LECs. White dashed line: VE-cadherin-negative junctions; white arrow: button-like junctions; yellow arrow: zipper-like junctions. Two-tailed unpaired Student’s t -test was performed to compare PA versus PA + DHA/EPA/ML-7-treated groups at the same time points. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Primary antibodies include CD36 (proteintech,18836–1-AP), VEGFC (proteintech,67116–1-Ig), VEGFR3 (R&D, AF743), ApoB48 (ThermoFisher, MA5-35458), MTTP (ThermoFisher, PA5-102474,), MLCK (ThermoFisher Scientific, PA5100908), GPR120 (Santa Cruz, sc-9901), p-MLC2 (CST, 95777S, Thr18/Ser19), GAPDH (Bioworld, AP0063), β-actin (Bioworld, AP0060) and β-Tubulin (Bioworld, AP0064).

    Techniques: Inhibition, Expressing, Two Tailed Test